Additional file 1 of FOXC1 promotes HCC proliferation and metastasis by Upregulating DNMT3B to induce DNA Hypermethylation of CTH promoter

Additional file 1: Supplementary Table S1. List of genes differentially expressed in Huh7-FOXC1 versus Huh7-control cells using a human amino acid metabolism PCR array. Supplementary Table S2. List of genes differentially expressed in MHCC97H-shFOXC1 versus MHCC97H-shcontrol cells using a human amino acid metabolism PCR array. Supplementary Table S3. Correlation between CTH methylation and clinicopathological characteristics of HCCs in two independent cohorts of human HCC tissues. Supplementary Table S4. Correlation between DNMT3B expression and clinicopathological characteristics of HCCs in two independent cohorts of human HCC tissues. Supplementary Table S5. Correlation between 8-OHdG expression and clinicopathological characteristics of HCCs in two independent cohorts of human HCC tissues. Supplementary Table S6. Correlation between pELK1 expression and clinicopathological characteristics of HCCs in two independent cohorts of human HCC tissues. Supplementary Table S7. Primer sequences used in the study. Supplementary Table S8. Knockdown shRNA sequences used in this study.

附加文件1：补充表S1。基于人类氨基酸代谢聚合酶链反应（PCR）芯片，鉴定Huh7-FOXC1细胞与Huh7-对照细胞之间的差异表达基因列表。补充表S2。基于人类氨基酸代谢聚合酶链反应（PCR）芯片，鉴定MHCC97H-shFOXC1细胞与MHCC97H-sh对照细胞之间的差异表达基因列表。补充表S3。在两独立人肝细胞癌（Hepatocellular carcinoma, HCC）组织队列中，分析胱硫醚γ裂解酶（CTH）甲基化与肝细胞癌患者临床病理特征的相关性。补充表S4。在两独立人肝细胞癌（HCC）组织队列中，分析DNA甲基转移酶3B（DNMT3B）表达与肝细胞癌患者临床病理特征的相关性。补充表S5。在两独立人肝细胞癌（HCC）组织队列中，分析8-羟基脱氧鸟苷（8-OHdG）表达与肝细胞癌患者临床病理特征的相关性。补充表S6。在两独立人肝细胞癌（HCC）组织队列中，分析磷酸化ELK1（pELK1）表达与肝细胞癌患者临床病理特征的相关性。补充表S7。本研究中使用的引物序列列表。补充表S8。本研究中使用的敲低短发夹RNA（shRNA）序列列表。