circAMN1/miR-205_R-1/SLC39A8 axis regulates ferroptosis in endometrial epithelial cells and regulates the occurrence of retained placenta in cows

In this study, we performed RNA-sequencing to characterize the expression profiles of circRNAs in caudal vein blood samples of postpartum Holstein cows whose fetal membranes were discharged normally or retained to identify retained placenta (RP)-related genes and evaluate their molecular mechanisms. Overall design: Comparative gene expression profiling analysis of RNA-seq data for caudal vein blood samples of postpartum Holstein cows whose fetal membranes were discharged normally (NC) or retained (n=3).

本研究通过RNA测序（RNA-sequencing），对胎膜正常排出与滞留的产后荷斯坦奶牛的尾静脉血液样本中的环状RNA（circRNAs）表达谱进行系统表征，旨在筛选胎衣不下（RP）相关基因并解析其潜在分子机制。
实验设计概述：本研究对胎膜正常排出组（normal control, NC组，n=3）与胎膜滞留组（n=3）的产后荷斯坦奶牛尾静脉血液样本的RNA测序数据开展比较基因表达谱分析。