Reprogram-Seq: A platform for single-cell combinatorial reprogramming [III]

Reprogram-Seq leverages organ-specific cell atlas data with single-cell perturbation and computational analysis to predict, evaluate, and optimize TF combinations that reprogram a cell type of interest. Overall design: Focusing on the cardiac system, we performed Reprogram-Seq on P0 mouse heart cells to generate a reference transcriptomic map. Based on the reference map, we selected TF candidates and tests 1000s of TF cocktails for direct lineage conversion by scRNA-Seq. This series includes uninfected, non-transformed MEFs.

重编程测序（Reprogram-Seq）借助器官特异性细胞图谱数据、单细胞扰动实验与计算分析手段，对可介导目标细胞类型重编程的转录因子（Transcription Factor, TF）组合进行预测、评估与优化。实验整体设计：本研究以心脏系统为研究对象，对出生后0天（P0）的小鼠心肌细胞开展重编程测序实验，以构建参考转录组图谱。基于该参考转录组图谱，研究团队筛选得到候选转录因子（TF），并通过单细胞RNA测序（scRNA-Seq）对数千种用于直接谱系转换的转录因子组合方案开展测试。本数据集系列包含未感染、未转化的小鼠胚胎成纤维细胞（Mouse Embryonic Fibroblasts, MEFs）。