RNA seq-based transcriptional profiling of intracellular Brucella abortus and Brucella abortus-infected murine macrophages

We describe the isolation of sufficient Brucella abortus RNA from primary host cell environment using modified reported methods for microarray analysis, and simultaneously characterize the transcriptional profiles of intracellular B. abortus and bone marrow-derived macrophages (BMM) from BALB/c mice at 24 h (replicative phase) post-infection.

本研究通过改良已报道的基因芯片（microarray）分析方法，从原代宿主细胞环境中分离得到足量的牛布鲁氏菌（Brucella abortus）RNA，并同时表征了感染后24小时（处于复制增殖期）的BALB/c小鼠来源的胞内牛布鲁氏菌与骨髓来源巨噬细胞（BMM）的转录谱。