Genicanthus semifasciatus Genome sequencing. Genicanthus semifasciatus

Samples of Genicanthus semifasciatus (voucher no. 340) were purchased from local aquarium and originally imported from Cebu, Philippines. Total genomic DNA was extracted from muscle tissue. Paired-end genomic library was constructed by using Illumina TruSeq DNA PCR-Free Library Preparation Kits and then performed next generation sequencing by Miseq splatform (2X300bp).

凭证标本编号为340的半纹月蝶鱼（Genicanthus semifasciatus）样本购自当地水族店，其原始引种来源为菲律宾宿务。从该样本的肌肉组织中提取总基因组DNA，采用Illumina TruSeq DNA无PCR建库试剂盒构建双端基因组文库，随后使用MiSeq测序平台（2×300bp模式）进行下一代测序。