Paired-end RNA-seq data from 12 ascites-derived organoid samples of ovarian cancer patients

This dataset contains paired-end RNA sequencing data from 12 ovarian cancer patient Ascites derived organoid samples. Samples were collected, processed, and sequenced to study transcriptomic changes associated with Doppel expression, epithelial-to-mesenchymal transition, and tumor dissemination. Each sample consists of two raw FASTQ files: - **R1**: Forward reads (`_1.fq.gz`) - **R2**: Reverse reads (`_2.fq.gz`) File Naming Convention Files are as follows: ‘PtXX_1.1.fq.gz’ → Forward read (R1) for patient XX ‘PtXX_1.2.fq.gz’ → Reverse read (R2) for patient XX Example: - Pt24AsOr1_1.fq.gz→ Forward reads from patient 24 - Pt24AsOr1_2.fq.gz→ Reverse reads from patient 24 Sample List: Please see the metadata file Data Summary · Total samples: 12 (24 FASTQ files; paired end). · Organism: Homo sapiens. · Tissue source: Ovarian tumor ascites-derived cells. · Sequencing platform: Illumina NovaSeq 6000. · Read length: 2 × 150 bp. · Data type: Raw FASTQ (gzip-compressed) Methods Summary RNA was extracted from ascites-derived organoids using combined Trizol and Qiagen RNase easy mini kit. RNA quality was assessed using bioanalyzer, and libraries were prepared using TruSeq Stranded mRNA Library Prep Kit. Sequencing was performed on an Illumina NovaSeq 6000 platform with paired end reads of (2 × 150 bp). Usage Notes · Use the forward (R1) and reverse (R2) files together for alignment and downstream analyses. · FASTQ files are untrimmed; adapters and low-quality bases should be removed prior to analysis. · Recommended Aligner: HISAT2. Contact For questions regarding this dataset, please contact: Dr. Taslim A. Al-Hilal, Associate Professor, Department of Molecular Pharmaceutics, University of UTAH, Salt Lake City, UTAH, Email: [e-mail]本数据集包含12例卵巢癌患者腹水来源类器官样本的双端RNA测序（paired-end RNA sequencing）数据。 样本经采集、处理与测序，旨在探究与Doppel表达、上皮间质转化（epithelial-to-mesenchymal transition）及肿瘤播散相关的转录组学变化。 每份样本包含两个原始FASTQ文件： - **R1**：正向读段（`_1.fq.gz`） - **R2**：反向读段（`_2.fq.gz`） 文件命名规范 文件命名规则如下： ‘PtXX_1.1.fq.gz’ → 患者XX的正向读段（R1） ‘PtXX_1.2.fq.gz’ → 患者XX的反向读段（R2） 示例： - Pt24AsOr1_1.fq.gz → 患者24的正向读段 - Pt24AsOr1_2.fq.gz → 患者24的反向读段 样本列表：请参阅元数据文件 数据概况 · 总样本量：12例（共24个FASTQ文件，为双端测序数据） · 物种：智人（Homo sapiens） · 组织来源：卵巢肿瘤腹水来源细胞 · 测序平台：Illumina NovaSeq 6000 · 读长：2 × 150 bp · 数据类型：原始FASTQ（gzip压缩格式） 实验方法概述 采用Trizol与Qiagen RNase easy mini试剂盒联合提取腹水来源类器官的RNA；使用生物分析仪评估RNA质量；通过TruSeq Stranded mRNA Library Prep Kit构建测序文库；在Illumina NovaSeq 6000平台上开展双端测序，读长为2 × 150 bp。 使用说明 · 需将正向（R1）与反向（R2）文件联合用于序列比对及下游分析 · FASTQ文件未经过修剪，分析前需去除接头序列与低质量碱基 · 推荐比对工具：HISAT2 联系方式 若有关于本数据集的疑问，请联系： Taslim A. Al-Hilal博士 副教授 犹他大学分子药剂学系 美国犹他州盐湖城 邮箱：[e-mail]