Expression data of transfected SKOV-3 cells before and after cisplatin treatment

Sensitivity to cisplatin is increased in SKOV-3 cells after transfecting the cells with unphosphorylatable PEA-15 (PEA-15AA) but not in cells transfected with the empty vector and phosphomimetic PEA-15 (PEA-15DD). In order to investigate the regulation of the underlying genes that increased the sensitivity to cisplatin after transfection with PEA-15AA, a small and well-annotated Clariom S gene microarray was performed. SKOV-3 cells were transfected with PEA-15-HA- (empty vector, EV), PEA-15AA- or PEA-15DD-containing plasmids. Twenty-four hours after transfection, cells were treated with 15 µM cisplatin (EC50 of cisplatin for EV-transfected cells over 48 hours) for 24 hours and then the total RNA of the cells was extracted. ClariomS assay was performed by Life and Brain GmbH, Bonn, Germany.

当使用非磷酸化型PEA-15（PEA-15AA）转染SKOV-3细胞后，该细胞对顺铂的敏感性显著升高；而转染空载体与磷酸化模拟型PEA-15（PEA-15DD）的细胞则未出现该变化。为探究转染PEA-15AA后提升顺铂敏感性的潜在调控基因，本研究采用小型且注释完善的Clariom S基因芯片（Clariom S gene microarray）开展实验。实验中将SKOV-3细胞分为三组，分别转染携带PEA-15-HA标签的空载体（empty vector, EV）、PEA-15AA或PEA-15DD的质粒。转染24小时后，使用15 μM顺铂（针对空载体转染细胞48小时的顺铂半数效应浓度EC50）处理细胞24小时，随后提取细胞总RNA。本次ClariomS检测由德国波恩的Life and Brain GmbH公司完成。