Methyl-Micro-C

Simultaneous measurement of epigenetic signatures from the same cell and tissue provides significant benefits for research, especially when resources are limited, and precise analysis is essential. Here, we report a technique called Methyl-Micro-C to simultaneously map chromatin accessibility, interaction, and DNA methylation in the same sample. Methyl-Micro-C enhances the resolution of chromatin interaction and the coverage of CpGs by combining MNase-mediated fragmentation with enzymatic conversion. This technique is relatively straightforward, making it easy for researchers to apply. It also allows for the profiling of three-dimensional epigenomes with consistent chromatin accessibility, interaction, and DNA methylation signals in a cost-efficient manner. Overall design: We developed the Methyl-Micro-C method and compared chromatin accessibility, interaction, and DNA methylation using Micro-C, EM-seq, NOMe-seq, and NOMe-Hi-C data.

对同一细胞与组织的表观遗传特征开展同步检测，可为研究带来显著优势，尤其在研究资源有限且需实施精准分析的场景中尤为关键。本研究报道了一种名为Methyl-Micro-C的技术，可在同一样本中同步绘制染色质可及性、染色质相互作用以及DNA甲基化图谱。该技术通过结合微球菌核酸酶（MNase）介导的片段化与酶促转化，提升了染色质相互作用的分辨率与CpG位点的覆盖度。其操作相对简便，便于研究人员推广应用；同时能够以高性价比的方式，获取染色质可及性、相互作用与DNA甲基化信号一致性良好的三维表观基因组数据。实验整体设计：我们开发了Methyl-Micro-C方法，并借助Micro-C、EM-seq、NOMe-seq以及NOMe-Hi-C测序数据，对染色质可及性、相互作用与DNA甲基化水平进行了对比分析。