The effect of Anaerostipes caccaei and Bifidobacterium bifidum on epithelial cells of colon

The purpose of this study is to determine whether cross feeding relationships can contribute to the alleviation of colitis. The two strains used in this study are Anaerostipes caccae and Bifidobacterium bifidum. Both strains are clearly present in significant amounts in the intestines of healthy humans. A. caccae is a butyrate-producing bacterium that uses lactic acid and acetate to produce butyrate. On the other hand, B. bifidum is known to produce lactic acid and acetate. The purpose of this study is to confirm the effect of the ecological network between the two strains on the improvement of colitis, particularly in terms of protecting intestinal epithelial cells. RNA-seq profiling of colonic epilthelial cells of DSS-induced murine colitis mouse with A. caccae and B. bifidum treatment

本研究旨在探究交叉喂养关系是否有助于缓解结肠炎。本次实验采用的两株菌株分别为粪便厌氧棍状杆菌（Anaerostipes caccae）与两歧双歧杆菌（Bifidobacterium bifidum），二者在健康人体肠道内均以显著丰度存在。其中，粪便厌氧棍状杆菌为产丁酸菌，可利用乳酸与乙酸合成丁酸；而两歧双歧杆菌则可产生乳酸与乙酸。本研究旨在确认两株菌株间的生态网络对结肠炎的改善作用，尤其是在保护肠上皮细胞方面。实验对经葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎模型分别施以粪便厌氧棍状杆菌与两歧双歧杆菌干预，并对其结肠上皮细胞开展RNA测序（RNA-seq）分析。