Characterization of differential gene expression pattern in control and microglia depleted retinas during retinal vascular development in mice. Characterization of differential gene expression pattern in control and microglia depleted retinas during retinal vascular development in mice

This study reports the comprehensive gene expression profiling of postnatal day (P) 10 control diet (Control) and Csf1r antagonist (PLX5622, Plexicon Inc. Berkeley, CA) diet fed microglia deplted retinas (referred as PLX5622) during development. Further functional enrichment analysis revealed down-regulation of biological processes linked to phagocytosis and innate immune signaling. Overall design: Retinal mRNA profiles of P10 control and microglia depleted (PLX5622) mice were generated by deep sequencing, n=4/genotype, using Illumina Nextseq 2000, and the sequencing target was 25 million reads per samples.

本研究报道了发育阶段内，分别喂食对照组饲料（Control组）与集落刺激因子1受体（Colony-stimulating factor 1 receptor, CSF1R）拮抗剂PLX5622（由美国加利福尼亚州伯克利市Plexicon公司生产）饲料的产后第10天（postnatal day, P10）小鼠的小胶质细胞（microglia）缺失视网膜样本（以下简称PLX5622组）的全基因表达谱。进一步功能富集分析显示，与吞噬作用及先天免疫信号通路相关的生物学过程出现显著下调。实验设计：本研究采用Illumina Nextseq 2000测序平台，对P10野生型对照组与小胶质细胞缺失（PLX5622处理）小鼠的视网膜mRNA进行深度测序，每组设置4个生物学重复，每个样本的测序目标读段数为2500万。