Human monocyte-derived macrophage response to Aspergillus fumigatus

To investigate the influence of Aspergillus fumigatus on iron regulation in macrophages, we obtained macrophages in culture from human derived monocytes and co-cultured the monocyte-derived macrophages with Aspergillus conidia at a 1:1 ratio. We collected samples at 0, 2, 4, 6 and 8 hours and extracted RNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of control macrophages and macrophage co-cultured with Aspergillus fumigatus at five time points. Overall design: Comparative gene expression profiling analysis of RNA-seq data for control human monocyte-derived macrophage and human monocyte-derived macrophage co-cultured with Aspergillus fumigatus.

为探究烟曲霉（Aspergillus fumigatus）对巨噬细胞铁调节的影响，我们从人源单核细胞中体外培养获得巨噬细胞，并以1:1的比例将单核细胞来源的巨噬细胞与烟曲霉分生孢子进行共培养。我们分别于0、2、4、6和8小时五个时间点收集样本并提取RNA，随后基于对照巨噬细胞以及与烟曲霉共培养的巨噬细胞在五个时间点下的RNA-seq数据开展基因表达谱分析。实验整体设计：针对人源单核细胞来源的对照巨噬细胞，以及与烟曲霉共培养的人源单核细胞来源的巨噬细胞的RNA-seq数据开展对比基因表达谱分析。