PSPs-1 regulate LCA metabolism is association with the regulation of Lactobacillus reuteri

In this study, we identified a homogeneous polysaccharide from processed Polygonatum cyrtonema Hua that can increase the abundance of Lactobacillus reuteri. Polygonatum cyrtonema Hua is a herbal medicine with a long history of medicinal use in China, due to its irritancy, it is often used after repeated steaming and sun drying, and it has good therapeutic effects on ulcerative colitis. We discoveried a new polysaccharide, which was produced during the repeated steaming and drying process of Polygonatum cyrtonema Hua. We explored the mechanism by which PSP improved UC through metabolomics combined with 16S sequencing technology. Results indicated that PSP can improve LCA metabolism abnormalities and achieve therapeutic effects on UC by increasing the abundance of Lactobacillus reuteri.

本研究中，我们从经炮制处理的多花黄精（Polygonatum cyrtonema Hua）中分离得到一种均一多糖，该多糖可提升罗伊氏乳杆菌（Lactobacillus reuteri）的丰度。多花黄精是我国应用历史悠久的传统中药材，因其原生药材具有刺激性，常需经反复蒸晒后入药，且对溃疡性结肠炎（ulcerative colitis）具有良好的治疗作用。我们发现了一种新型均一多糖，该多糖系多花黄精在反复蒸晒炮制过程中生成。我们结合代谢组学（metabolomics）与16S测序技术（16S sequencing technology），探究了该均一多糖（PSP）改善溃疡性结肠炎的作用机制。研究结果表明，该均一多糖可通过提升罗伊氏乳杆菌的丰度，改善石胆酸（LCA）代谢异常，从而实现对溃疡性结肠炎的治疗作用。