sRNA and transcriptome sequencing of populus simonii × P. nigra

Cold tolerance is important for plants geographical distribution and surviving in extreme seasonal variations of climate. However, populus simonii × P. nigra shows wide adaptability and strong cold resistance. Transcriptional and post-transcriptional regulation of cold-responsive genes is crucial for cold tolerance in plants. To understand the roles of regulatory RNAs under cold stress in populus simonii × P. nigra, we constructed cDNA and small RNA libraries from leaf buds treated or not with 4 °C for 8 h for analysis.低温耐受性对于植物的地理分布以及在极端季节性气候波动中存活至关重要。然而，小叶杨×黑杨（Populus simonii × P. nigra）具有广泛的适应性与极强的低温抗性。植物低温响应基因的转录及转录后调控，对于其低温耐受性至关重要。为解析小叶杨×黑杨在低温胁迫下调控RNA的生物学功能，本研究分别从经4℃低温处理8小时与未经过低温处理的叶芽中构建了cDNA文库与小RNA文库，用于后续分析。