Dietary selenium levels affect selenoprotein expression and support the interferon-γ and IL-6 immune response pathways in mice [RNA-Seq]. Mus musculus

Mice were fed Se-deficient or Se-adequate diets for 6 weeks. Liver and lung tissue were harvested and processed for RNA-Seq, ribosome profiling, and microarray analysis. From these studies, we identified changes in mRNA levels and translation of selenoprotein genes and genes regulated by interferon-gamma. Cytokine profiles of serum indicated that interferon-gamma and IL-6 levels were increased in the Se-adequate mice relative to Se-deficient mice. Overall design: RNA-Seq analysis of liver tissue from mice fed Se-deficient or Se-adequate diets

本研究将小鼠分为两组，分别饲喂硒缺乏（Se-deficient）饲料与硒充足（Se-adequate）饲料，持续6周。随后采集两组小鼠的肝脏与肺组织，开展RNA测序（RNA-Seq）、核糖体谱分析（ribosome profiling）及微阵列（microarray）分析。通过上述实验，我们鉴定出硒蛋白基因以及受干扰素-γ（interferon-gamma）调控的基因的mRNA水平与翻译过程的变化。血清细胞因子谱分析结果显示，相较于硒缺乏组小鼠，硒充足组小鼠血清中的干扰素-γ与白细胞介素-6（IL-6）水平显著升高。整体实验设计：对饲喂硒缺乏或硒充足饲料的小鼠的肝脏组织进行RNA测序（RNA-Seq）分析。