ATAC-sequencing data for 923 enhancer deletion newborn mouse keratinocytes

We examined the ATAC-seq chromatin profiles of epidermis from newborn CRISPR-edited mice that have the 923 enhancer located within the Epidermial Differentiation Complex deleted. We examined two independent deletion lines, 923del and 923large, homozygous mutants, as well as wild type mice. We find overall there are not many differences in chromatin accessibility upon the loss of the 923 enhancer, though there are a few peaks of less accessible DNA within the EDC and more accessible DNA just outside the EDC. Overall design: Examination of accessible chromatin profiles by ATAC-seq in two 923 enhancer deletion lines and WT newborn mouse epidermis.

本研究分析了携带缺失表皮分化复合体（Epidermal Differentiation Complex，EDC）内923增强子（enhancer）的CRISPR（成簇规律间隔短回文重复序列）编辑新生小鼠表皮的转座酶可及性染色质测序（ATAC-seq）染色质图谱。本次研究共纳入两个独立的缺失品系：纯合突变体923del与923large，以及野生型小鼠。
研究结果显示，整体而言，923增强子缺失后染色质可及性（chromatin accessibility）并未出现显著差异，但在EDC内部存在少量DNA可及性降低的峰，而EDC外侧区域则存在更多DNA可及性升高的位点。
实验设计：通过ATAC-seq分析两个923增强子缺失品系与野生型（Wild Type, WT）新生小鼠表皮的染色质可及性图谱。