Liver macrophages inhibit the endogenous antioxidant response in obesity-associated insulin resistance [miRNA-Seq]

Liver Macrophages exacerbate oxidative stress induced by hepatic steatosis in obesity by blocking the endogenous anti-oxidant response. Kupffer cell miRNA profiles of wild type and obese mice at 9 and 14 weeks were generated by small-RNA sequencing, in replicates, using Illumina HiSeq 3000.

肝脏巨噬细胞（Liver Macrophages）通过阻断内源性抗氧化应答，加剧肥胖状态下肝脂肪变诱导的氧化应激。研究人员采用Illumina HiSeq 3000测序平台，通过小RNA测序（small-RNA sequencing）技术，对9周龄和14周龄的野生型小鼠与肥胖小鼠的库普弗细胞（Kupffer cell）miRNA表达谱进行了重复样本测序与构建。