Wheat transcriptome: Effect of synergistic interaction between Wheat Streak Mosaic Virus and Triticum Mosaic Virus

The effect of synergistic interaction between Wheat Streak Mosaic Virus (WSMV) and Triticum Mosaic Virus (TriMV) on wheat transcriptome was examined by performing RNA-Seq from singly and doubly infected wheat at collected at 9, 12, and 21 days postinoculation (dpi). A total of 31,754 differentially expressed genes (DEGs) were identified. Gene ontology and domain enrichment analysis of commonly expressed DEGs revealed important genes related to photosynthesis, stress, and defense. Synergistic interactions between WSMV and TriMV induced wheat genes involved in the TCA cycle, stress responses, and increased expression of bZIP, B3, and NAC transcription factors while having detrimental effects on starch biosynthesis, photosynthesis, and MADS-box transcription factors.

本研究通过对接种后9、12和21天（days postinoculation, dpi）的单接种与双接种小麦样本开展RNA测序（RNA-Seq），解析了小麦条纹花叶病毒（Wheat Streak Mosaic Virus, WSMV）与小麦花叶病毒（Triticum Mosaic Virus, TriMV）的协同互作对小麦转录组的调控效应。研究共鉴定得到31754个差异表达基因（differentially expressed genes, DEGs）；对共同差异表达基因的基因本体论（Gene ontology）与结构域富集分析显示，存在大量与光合作用、胁迫响应及免疫防御相关的核心基因。WSMV与TriMV的协同互作可诱导小麦参与三羧酸循环（TCA cycle）、胁迫应答的基因表达，并上调bZIP、B3及NAC转录因子的转录水平；同时会对淀粉生物合成、光合作用及MADS-box转录因子产生负面调控效应。