4D_Luciferase assay_let-7(0) on cdk-1 RNAi

Luciferase assay data used to quantify the duration of larval stages of animals of the indicated genotypes. The animals were fed E. coli OP50 bacteria or E. coli HT115 bacteria, either containing the insert-less L4440 parental RNAi vector (denoted “EV”) or an RNAi vector with an insert targeting the gene of interest (cdk-1 or grh-1 as a technical positive control). The file contains three sheets: Raw data: Contains the raw values measured by the luminometer (Berthold Technologies, Centro XS3 LB 960). Luminescence was measured every 10 min for 0.5 seconds. The luminometer was placed in a temperature-controlled incubator set to 18.5°C, which translated to an effective temperature of 22.5°C experienced by the animals inside the luminometer. Plate design: Position of animals of indicated genotypes in the 384-well luminometer plate. In let-7(0), lin-46(0); let-7(0) and the triple mutant animals, only wells containing unbalanced animals were labeled and considered for the analysis. Annotated data: List of animals of indicated genotypes with the times of hatch, molt entry and exit in hours. Animals excluded from the analysis are indicated by "0" in the "is_valid" column. let-7(0) HW2791 xeSi312 [eft-3p::luc::gfp::unc-54 3’UTR, unc-119(+)] IV; let-7(xe150) X; xeEx365 [let-7p::let-7::SL1_operon_GFP, unc-119 (+); Prab-3::mCherry; Pmyo-2::mCherry; Pmyo-3::mCherry] triple mut HW3543 lin-28(xe192) I; xeSi312 [eft-3p::luc::gfp::unc-54 3’UTR, unc-119(+)] IV; lin-46(ma385) V; let-7(xe150) X; xeEx365 [let-7p::let-7::SL1_operon_GFP, unc-119 (+); Prab-3::mCherry; Pmyo-2::mCherry; Pmyo-3::mCherry] lin-46(0); let-7(0) HW3544 xeSi312 [eft-3p::luc::gfp::unc-54 3’UTR, unc-119(+)] IV; lin-46(ma385) V; let-7(xe150) X; xeEx365 [let-7p::let-7::SL1_operon_GFP, unc-119 (+); Prab-3::mCherry; Pmyo-2::mCherry; Pmyo-3::mCherry]