High-throughput sequencing (HTS) data of plasmid library subjected to Cas12a cleavage

Cas12a (formerly Cpf1) is an RNA-guided endonuclease in the CRISPR-Cas immune system that can be easily programmed for genome editing. To understand the native cleavage specificity and cleavage activity of Cas12a, we employed high-throughput <i>in vitro</i> cleavage assays using a plasmid library (pLibrary). We tested the cleavage activity of three Cas12a orthologs – FnCas12a, LbCas12a and AsCas12a. For each Cas12a ortholog, we used three different crRNA sequences and corresponding target libraries (pLibrary), referred to as pLibrary PS4, EMX1 and CCR5. The data set is the high-throughput sequencing (HTS) reads of pLibrary subjected to cleavage by different Cas12a orthologs. The README file contains details about the content of the HTS data set, and the key to the file names and corresponding samples.<br> This HTS data set supports the research article: "CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects". <br>Previous title: "Pervasive off-target and double-stranded DNA nicking by CRISPR-Cas12a". <br>Version 2: New data set for pLibrary CCR5 uploaded with updated "README" file (v2).<br>Version 3: Data set corrected for pLibrary PS4 with updated "README" file (v3).<br><br>Version 4: "README" file (v4) and reference updated with new title and link to publication.

Cas12a（原名Cpf1）是CRISPR-Cas免疫体系中一种RNA引导的核酸内切酶，可被轻松编程用于基因组编辑。为了解Cas12a的天然切割特异性与切割活性，我们采用质粒文库（pLibrary）开展了高通量<i>体外</i>切割实验。我们测试了三种Cas12a同源蛋白——FnCas12a、LbCas12a和AsCas12a的切割活性。对于每种Cas12a同源蛋白，我们使用了三种不同的crRNA序列及对应的靶标文库（pLibrary），即pLibrary PS4、EMX1和CCR5。本数据集为经过不同Cas12a同源蛋白切割后的pLibrary的高通量测序（HTS）读数。README文件包含HTS数据集内容的详细信息，以及文件名与对应样本的说明。<br>本HTS数据集支持以下研究文章："CRISPR-Cas12a具有广泛的脱靶效应和双链DNA切口效应"。<br>此前标题："CRISPR-Cas12a的普遍脱靶效应与双链DNA切割"。<br>版本2：上传了pLibrary CCR5的新数据集及更新后的README文件（v2）。<br>版本3：修正了pLibrary PS4的数据集，并更新了README文件（v3）。<br><br>版本4：更新了README文件（v4）及参考文献，包含新标题和论文链接。