Sirt1-microRNA profile

Sirt1, a NAD-dependent deacetylase, has been shown involved in many complex biological functions. We previously demonstrated that Sirt-1 gain of function is neuroprotective in a model of neurodegenenration. We recently found that Sirt1 loss of function (Sirt1delta mouse) impairs learning, memory, and synaptic plasticity. We used microarray screening to identify potential miRNAs that may be regulated by Sirt1 and involved in learning and memory. Total RNA from the hippocampus of SIRT1delta and control mice was extracted and used for microRNA expression level evaluation. Two group experiment, in duplicates. Each sample is compared to a common reference sample, comprised of an equal mixture of all 4 experimental samples.沉默信息调节因子1（Sirt1）是一类烟酰胺腺嘌呤二核苷酸（Nicotinamide Adenine Dinucleotide, NAD）依赖性去乙酰化酶，现已被证实参与诸多复杂的生物学功能。我们前期研究证实，在神经退行性疾病模型中，Sirt1功能获得可发挥神经保护作用。近期我们发现，Sirt1功能缺失（Sirt1delta小鼠）会损伤学习、记忆能力与突触可塑性。本研究采用微阵列芯片（microarray）筛选技术，鉴定可能受Sirt1调控并参与学习记忆过程的潜在微小RNA（microRNA, miRNA）。我们提取了Sirt1delta小鼠与对照小鼠海马组织的总RNA，用于miRNA表达水平的检测与评估。本实验分为两组，每组设置生物学重复；每个实验样本均与由全部4份实验样本等比例混合制备的通用参考样本进行比对。