Profiling histone modifications in clinical archived formalin fixed paraffin embedded samples

Here, we present FACT-Seq, a highly sensitive method to decode the histone modifications in FFPE tissues. The FACT-Seq generates the high-quality chromatin profiles from both active and silent histone modifications with 1000 nuclei purified from a single FFPE tissue section, and reveals the disease-specific super enhancers in the FFPE archived human colorectal and human glioblastoma multiforme cancer tissue. In summary, the approach allows decoding the histone modifications that regulate gene expression in archival FFPE tissues with high sensitivity, in order to better understand epigenetic regulation in cancer and precision medicine. Overall design: FACT-Seq of mouse FFPE kidney and clinical archived CRC and GBM.

本研究报道了FACT-Seq这一高灵敏度组蛋白修饰解析方法，可用于福尔马林固定石蜡包埋（FFPE, Formalin-Fixed Paraffin-Embedded）组织的组蛋白修饰检测。该方法仅需从单个FFPE组织切片中纯化得到的1000个细胞核，即可获取覆盖活性与沉默型组蛋白修饰的高质量染色质图谱，并能在存档的人类结直肠癌（CRC, Colorectal Cancer）与多形性胶质母细胞瘤（GBM, Glioblastoma Multiforme）组织中揭示疾病特异性超级增强子。综上，该方法可高灵敏度地解析存档FFPE组织中调控基因表达的组蛋白修饰，为癌症与精准医学领域的表观遗传调控研究提供助力。
实验设计概述：针对小鼠FFPE肾脏组织以及临床存档的CRC与GBM样本开展FACT-Seq检测。