RNA-seq of detecting the effect of NS5 for neurogenesis.. RNA-seq of detecting the effect of NS5 for neurogenesis.

The RNA-seq data provided evidence that ZIKV-NS5 repressed genes transcription, thus disrupting the neurogenesis of human NPCs. Overall design: overexpressed ZIKV-NS5 in hNPCs and collected cells 48 hours post infection by lentivirus. And hNPCs in control groups were infected by lentivirus that packaged vectors.

RNA测序（RNA-seq）数据证实，寨卡病毒NS5蛋白（ZIKV-NS5）可抑制基因转录，进而破坏人类神经前体细胞（human NPCs）的神经发生过程。
实验设计概述：通过慢病毒在人类神经前体细胞（hNPCs）中过表达ZIKV-NS5，并于感染后48小时收集细胞；对照组的人类神经前体细胞则接受仅包装空载体的慢病毒感染。