Histopathological studies of red palm weevil Rhynchophorus ferrugineus, (Olivier) larvae and adults to evaluate certain nano pesticides

Abstract Rhynchophorus ferrugineus (RPW) (Olivier) (Coleoptera: Curculionidae) is one of the most destructive pest of palm according to dozens of literature, a lot of effort have been made since three decades up to date to solve this problem, one of newest solution raised is using nano pesticides. Imidacloprid and Chloropyrophos and their nano form were tested against 10 days-old larvae and newly emerged adults of the red palm weevil. The pesticides had toxicological and pathological effects on Rh. ferrugineus larvae and adults. The toxicity effect of nano chloropyrophos was more than others. The damage included vaculation of cytoplasm, analyzes and destroyed nuclei of the epithelial cells. The larvae were more sensitive in the total damages in comparison with adults. This investigation is the first record to the histopathological effects of nano pesticides.

摘要：红棕象甲（*Rhynchophorus ferrugineus*，简称RPW）（Olivier）隶属于鞘翅目（Coleoptera）象甲科（Curculionidae），据数十篇文献记载，其为棕榈科植物最具破坏性的害虫之一。近三十年来，科研人员为解决该虫害问题已投入大量研究工作，其中一项新兴防治方案为纳米农药（nano pesticides）。本研究针对红棕象甲10日龄幼虫及初羽化成虫，测试了吡虫啉（Imidacloprid）、毒死蜱（Chloropyrophos）及其纳米剂型的杀虫效果。结果显示，供试农药对红棕象甲幼虫与成虫均具有毒理学与病理学效应，其中纳米毒死蜱的毒性效果优于其余供试药剂。药剂造成的损伤包括细胞质空泡化、上皮细胞的细胞核裂解与破坏。相较于成虫，幼虫对总损伤的敏感性更高。本研究首次记录了纳米农药对红棕象甲的组织病理学效应（histopathological effects）。