Density of IRS1 immunostaining in paired colonic epithelium, primary colorectal cancer and synchronous liver metastasis.

Density values (mean ± SEM), normalized per area size in square pixels, were obtained by digital analysis using ImageJ software (http://rsbweb.nih.gov/ij/) for IRS1 staining in: bottom versus top colonic crypt epithelium (respectively 11 and 10 areas); total colonic epithelium (78 areas) versus primary CRC (84 areas); total colonic epithelium (78 areas) versus liver metastasis (84 areas); primary CRC versus liver metastasis (84 areas). Only the differences between total colonic epithelium and liver metastasis and between primary CRC and liver metastasis are significant (P<0.01).
*Number of examined areas, CRC: colorectal cancer.

以每平方像素面积归一化后的密度值（平均值±标准误），通过ImageJ软件（官网地址：http://rsbweb.nih.gov/ij/）进行数字化分析获取，检测对象为IRS1染色样本，分组对比情况如下：结肠隐窝上皮底部与顶部（分别对应11和10个观测区域）；结肠总上皮（78个区域）与原发性结直肠癌（CRC）组织（84个区域）；结肠总上皮（78个区域）与肝转移灶（84个区域）；原发性结直肠癌组织与肝转移灶（84个区域）。仅结肠总上皮与肝转移灶、原发性结直肠癌与肝转移灶之间的差异具有统计学显著性（P<0.01）。
* 观测区域数量说明：CRC即结直肠癌（colorectal cancer）。