File S1 - Genetic Heterogeneity in a Large Cohort of Indian Type 3 von Willebrand Disease Patients

Tables S1–S3. Table S1: Primer sequence for screening known Arginine hot spot regions of VWF. Blue coloured font indicates artificial restriction site has been introduced by using a mismatched nucleotide. The bold font in the oligonucleotides represents a mismatched nucleotide. Table S2: Mutation detection by PCR-RFLP for Arginine hot spot mutations. R- arginine, *- stop codon. Adapted from Baronciani et al., 2000. Table S3: Primers used for sequencing are shown in table.
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附表S1至S3。
附表S1：用于筛查血管性血友病因子（von Willebrand factor, VWF）已知精氨酸热点区域的引物序列。蓝色字体表示通过引入错配核苷酸以构建人工限制性酶切位点；寡核苷酸序列中的加粗字体代表错配核苷酸。
附表S2：针对精氨酸热点突变的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）突变检测方法。其中R代表精氨酸，*代表终止密码子。本内容改编自Baronciani等2000年的研究。
附表S3：本研究所用测序引物列于本表中。
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