Loss of Zfp335 triggers cGAS/STING-dependent apoptosis of post-B-selection pre-T cells [RNA-seq]

The transcription factor Zfp335 is esstential for the survival of post-B-selection DN4 thymocytes. We utilized RNA-seq to profile the alterations to the transcriptional profiles of Zfp335-deficient DP thymocytes. DP thymocytes were FACS-sorted (Live Lin- CD4+ CD8+) from Zfp335-+/+ E8III-cre or Zfp335-fl/fl E8III-cre positive 7 week old female mice. Each sample was from one individual mouse. Following sorting, total RNA was isolated and libraries were prepared and sequenced at BGI genomics.