Aspartoacylase-dependent transcriptome changes in immortalized brown adipocytes

Here we investigated the effect of stable knock-down of the NAA-catabolizing enzyme, Aspartoacylase (Aspa), on global gene expression in a brown adipocyte cell line. Immortalized brown adipogenic cells (iBACs) with stable Aspa knock-down and control cells were grown until confluence and then differentiated. Cells were harvested on day 7. Experiment was done in triplicates with three different passages.

本研究探究了NAA代谢酶天冬氨酸酰基酶（Aspartoacylase, Aspa）的稳定敲低对棕色脂肪细胞系全局基因表达的影响。将稳定敲低Aspa的永生化棕色脂肪生成细胞（immortalized brown adipogenic cells, iBACs）与对照细胞培养至细胞汇合态，随后诱导其分化。于分化第7天收集细胞。本实验设置三次生物学重复，且采用三个不同传代批次的细胞完成。