Durable photobioreactor antibiofouling coatings for microalgae cultivation by photoreactive poly(2,2,2-trifluoroethyl methacrylate)

To improve the durability of the photobioreactor antibiofouling surface for microalgal cultivation, a series of photoreactive poly(2,2,2-trifluoroethyl methacrylate) (PTFEMA) were successfully synthesized and used to modify ethylene-vinyl acetate (EVA) films by a surface coating and UV light grafting method. Fourier transform infrared (FT-IR) spectra, X-ray photoelectron spectroscopy analysis (XPS) and fluorescence microscopy results indicated that PTFEMA were fixed successfully onto the EVA film surface through a covalent bond. During the microalgal adhesion assay, the number of EVA-PTFEMA film-adhered microalgae was 41.4% lower than that of the EVA film. Moreover, the number of microalgae attached to the EVA-PTFEMA film decreased by 61.7% after cleaning, while that of EVA film decreased by only 49.1%. It was found that the contact angle of EVA-PTFEMA film surface increased, and remained stable when immersed in acid and alkali solution for up to 90 days.HIGHLIGHTSDurable photobioreactor antibiofouling surfaces for microalgal cultivation were prepared successfully.The contact angle of antibiofouling coating surface remained stable in acid and base environment for 90 days.The attached microalgae on antibiofouling surface decreased 41.4% than those of unmodified surface.The attached microalgae on antibiofouling surface could be cleaned by 61.7% through changing the flow velocity of microalgal suspension. Durable photobioreactor antibiofouling surfaces for microalgal cultivation were prepared successfully. The contact angle of antibiofouling coating surface remained stable in acid and base environment for 90 days. The attached microalgae on antibiofouling surface decreased 41.4% than those of unmodified surface. The attached microalgae on antibiofouling surface could be cleaned by 61.7% through changing the flow velocity of microalgal suspension.

为提升用于微藻培养的光生物反应器抗生物污染表面的耐用性，本研究成功合成了一系列光反应性聚甲基丙烯酸-2,2,2-三氟乙酯（poly(2,2,2-trifluoroethyl methacrylate)，PTFEMA），并通过表面涂覆与紫外光接枝法将其用于改性乙烯-醋酸乙烯酯（ethylene-vinyl acetate, EVA）薄膜。傅里叶变换红外光谱（Fourier transform infrared, FT-IR）、X射线光电子能谱（X-ray photoelectron spectroscopy, XPS）分析及荧光显微镜观测结果表明，PTFEMA通过共价键成功固定于EVA薄膜表面。微藻粘附实验显示，EVA-PTFEMA薄膜表面粘附的微藻数量较纯EVA薄膜降低41.4%。此外，经清洗处理后，EVA-PTFEMA薄膜表面附着的微藻数量减少61.7%，而纯EVA薄膜仅减少49.1%。研究发现，EVA-PTFEMA薄膜表面的接触角有所提升，且在酸碱溶液中浸泡90天仍可保持稳定。

### 研究亮点
1. 成功制备了用于微藻培养的耐用型光生物反应器抗生物污染表面；
2. 该抗生物污染涂层表面的接触角在酸碱环境中可稳定维持90天；
3. 抗生物污染表面粘附的微藻数量较未改性表面降低41.4%；
4. 通过调控微藻悬浮液的流速，可清除抗生物污染表面61.7%的附着微藻。