Profiling study of B cells from lymph nodes in miR-146a over-expression transgenic mice. Mus musculus

B cells were isolated from wild type and miR-146a transgenic mice, and analyzed using microarray assay.WT1 and WT2 were samples repeats, so were TG1 and TG2 . Overall design: Each probe ID of a mouse transcript was ranked by fold change from up to down-regulated comparing to WT control. Sylamer miRNA seed enrichment analysis was performed with Markov correction as described (Nature Method 2008). WT vs. TG.

从野生型（wild type）及miR-146a转基因小鼠中分离B细胞，并通过微阵列（microarray）检测进行分析。WT1与WT2为生物学重复样本，TG1与TG2同理。实验设计概述：以野生型对照组为参照，将小鼠转录本的各探针ID（probe ID）按照表达倍数变化（fold change）从上调至下调进行排序。采用经马尔可夫校正的Sylamer miRNA种子序列富集分析方法，操作流程参照2008年《Nature Method》中所述内容。本次分析的比较组别为野生型（WT）与转基因型（TG）。