Metagenomic Analysis of The Composition, Abundance and Profile of Human Stool Enveloped and Extracellular Vesicles-encapsulated Nonenveloped Virus.

This dataset contains viral metagenomic sequencing clean reads data derived from human stool samples. It comprises 6 DNA samples and 6 RNA samples, categorized into two groups: PS-positive and Viral-like particles (VLPs). The DNA samples labeled D1_PS to D6_PS are part of the PS-positive group, while samples labeled D1 to D6 represent the VLPs group. In the RNA category, samples labeled A1_PS to A6_PS belong to the PS-positive group, and samples labeled A1 to A6 correspond to the VLPs RNA dataset.This metagenomic dataset provides a comprehensive profile of human stool enveloped viruses and extracellular VLP-encapsulated nonenveloped viruses. We isolated Enveloped particles from human stool extracellular VLPs using a Phosphatidylserine (PS) affinity kit. Our viral metagenomics analysis revealed significant variations in virome composition and abundance between the PS-positive and VLPs viromes. These findings enhance our understanding of: (1) ecological interactions between enveloped viruses (EnVs) and extracellular vesicles (EVs); (2) PS-mediated environmental persistence mechanisms; and (3) risk assessment frameworks for enteric viral transmission.

本数据集包含源自人类粪便样本的病毒宏基因组测序clean reads数据。该数据集涵盖6个DNA样本与6个RNA样本，分为PS阳性组和病毒样颗粒（Viral-like particles, VLPs）组两类。标记为D1_PS至D6_PS的DNA样本属于PS阳性组，标记为D1至D6的样本则归为VLPs组；RNA类别中，A1_PS至A6_PS样本属于PS阳性组，A1至A6样本对应VLPs RNA数据集。本宏基因组数据集提供了人类粪便中包膜病毒及细胞外VLPs包裹的非包膜病毒的全面图谱。我们通过磷脂酰丝氨酸（Phosphatidylserine, PS）亲和试剂盒从人类粪便的细胞外VLPs中分离出包膜颗粒。病毒宏基因组分析显示，PS阳性组与VLPs组的病毒组在组成及丰度上存在显著差异。这些发现有助于深化对以下内容的理解：（1）包膜病毒（Enveloped viruses, EnVs）与细胞外囊泡（Extracellular vesicles, EVs）间的生态相互作用；（2）PS介导的环境持久性机制；（3）肠道病毒传播的风险评估框架。