Source Data and protocols for White, et al 2025- The pyrethroid insecticide deltamethrin disrupts neuropeptide and monoamine signaling pathways in the gastrointestinal tract

Here, we provide source data, a list of key resources, and manufacturer protocols used in the manuscript: https://doi.org/10.1101/2024.12.14.628386, which describes the toxicological outcomes of pyrethroid exposure in enteroendocrine cells and the gastroinestinal tract. An excel spreadsheet containing the source data and statistical output, used in figure generation, and organized by figure panel, are included as the "SourceDataandStatistics" file. A spreadsheet file of the raw CT values, and gene expression calcuations, for the indicated qPCR gene expression analysis is included in the "QPCRDataAnalysis" file. RNAseq analysis for this project is further described in: https://doi.org/10.1101/2024.12.14.628386; which includes all alignment files, methods, and statistical outputs. In addition, maufacturer's protocols used in the library preparation (NEBNext) and sequencing (Cbot) are included here. Metascape pathway analysis and differential gene expression source data, used in figure generation of the associted manuscript are included here as the indicated "DEGSourceData" and "MetascapePathwayAnalysis" files. A Key Resource Table, containing identifiers for additional protocols, assays, and reagents, used in the study is also included.本研究提供了本文稿中使用的原始数据、关键资源清单及厂商实验方案，相关信息详见https://doi.org/10.1101/2024.12.14.628386，该文献阐述了拟除虫菊酯暴露于肠内分泌细胞及胃肠道后的毒理学效应。 包含原始数据与统计分析结果、用于绘图且按图组编排的Excel电子表格，以"SourceDataandStatistics"文件形式提供。针对指定定量聚合酶链式反应（qPCR）基因表达分析的原始CT值及基因表达量计算结果电子表格，收录于"QPCRDataAnalysis"文件中。 本项目的RNA测序（RNA sequencing, RNA-seq）分析细节详见https://doi.org/10.1101/2024.12.14.628386，该文献包含所有比对文件、实验方法及统计分析结果。此外，本研究还提供了文库制备（NEBNext试剂盒）与测序（Cbot平台）所用的厂商实验方案。 本关联手稿绘图所用的Metascape通路分析及差异基因表达原始数据，分别以"DEGSourceData"和"MetascapePathwayAnalysis"文件形式提供。 本研究还附带一份关键资源表，其中收录了本研究所用的额外实验方案、检测方法及试剂的相关标识符。