Comparative gene expression analysis of biliary tract cancer cells under organoid culture and adherent culture.. Comparative gene expression analysis of biliary tract cancer cells under organoid culture and adherent culture.

Organoid culture is important for maintenance of epithelial cell characteristics, stemness, and tumorigenic activity of biliary tract cancer initiating cells. To investigate whether organoid culture maintain cancer stem cell properties of biliary tract cancer initiating cells, we compared the gene expression changes between organoid culture and adherent culture. Overall design: Using Agilent mouse genome array, we compared global gene expression profiles in biliary tract cancer initiating cells under organoid culture and adherent culture.

类器官（Organoid）培养对于维持胆道癌起始细胞的上皮细胞特性、干细胞干性（stemness）以及致瘤活性具有重要意义。为探究类器官培养是否可维持胆道癌起始细胞的癌症干细胞特性，本研究对比了类器官培养与贴壁培养（adherent culture）之间的基因表达变化。整体实验设计：本研究采用安捷伦（Agilent）小鼠基因组芯片（mouse genome array），对比了处于类器官培养与贴壁培养条件下的胆道癌起始细胞的全基因表达谱。