RNA-seq datasets for enhancer RNA quantification in "Enhancer RNAs predict enhancer-gene regulatory links and are critical for enhancer function in neuronal systems". RNA-seq datasets for enhancer RNA quantification in "Enhancer RNAs predict enhancer-gene regulatory links and are critical for enhancer function in neuronal systems"

This dataset contains total RNA sequencing results from rat embryonic cortical, hippocampal, and striatal neuronal cultures treated with either vehicle (complete Neurobasal media) or potassium chloride (10mM) for 1hr prior to RNA extraction. Overall design: This experiment contains 19 biological samples, each of which underwent directional, 75bp paired-end total RNA-seq on an Illumina NextSeq500 instrument. Samples were treated with neurobasal media (vehicle; samples V1C, V2C, V3C, V1H, V2H, V3H, V2S, V4S, V6S, V8S), or 10mM KCl (samples K1C, K2C, K3C, K1H, K2H, K3H, K1S, K5S, K7S) . The first letter of sample names denotes acute treatement (V = vehicle, K = KCl). The second digit denotes the unique replicate ID. The third digit denotes the region of cultured neuron (C = cortex, H = hippocampus, S = striatum). NEBnext rRNA depletion kit was used to remove ribosomal RNA to include both polyadenylated and non-polyadenylated RNA. Samples were sequenced across multiple lanes of the Illumina instrument, designated as L001, L002, L003, L004 in raw file names. Forward and reverse paired-end reads are denoted as "R1" or "R2" in raw file names.

本数据集包含经溶剂对照（完整Neurobasal培养基）或氯化钾（10mM）处理1小时后提取RNA的大鼠胚胎皮层、海马体及纹状体神经元培养物的总RNA测序结果。总体设计：本实验共包含19份生物学样本，所有样本均在Illumina NextSeq500测序仪上完成定向75bp双端总RNA测序。样本分为两组：一组以Neurobasal培养基作为溶剂对照（样本编号：V1C、V2C、V3C、V1H、V2H、V3H、V2S、V4S、V6S、V8S），另一组接受10mM氯化钾处理（样本编号：K1C、K2C、K3C、K1H、K2H、K3H、K1S、K5S、K7S）。样本命名规则如下：首字母代表处理方式（V代表溶剂对照，K代表氯化钾处理）；第二位数字为唯一重复标识；第三位字母代表培养神经元的组织来源区域（C代表皮层，H代表海马体，S代表纹状体）。本次实验使用NEBnext核糖体RNA去除试剂盒（NEBnext rRNA depletion kit）移除核糖体RNA，以同时覆盖聚腺苷酸化与非聚腺苷酸化RNA。原始测序文件的测序泳道编号为L001、L002、L003、L004，正向与反向双端读段分别以"R1"和"R2"标注于原始文件名中。