Expression in AtBBX32 Transgenic Soybean

Transgenic soybean plants expressing AtBBX32 are taller, and have more nodes, flowers, pods, and seed. The effects of expression of AtBBX32 are most evident in altering expression of a subset of genes in the early morning hours. In particular, AtBBX32 alters expression of the soybean clock genes TOC1 and LHY1 This experiment compares the response of AtBBX32 transgenic plants with a control plant under field grown conditions at five time points. Four lines, five timepoints and three biological replicates for a total of 60 samples. Of the four lines, three were AtBBX32 transgenic lines (of which two were high yielding [Event1 and Event2], one was not [Event4]), and a non-transgenic control event [A3244]. Sample times: 3AM, 6AM, 9AM, Noon, and 3PM (ZT21, ZT0, ZT3, ZT6 and ZT9) (Sunrise was at 6:08 AM and Sunset was at 8:08 PM) Development Stage and Tissue: Trifoliate leaf at R5 stage (80 days post-emergent) Each of the three bioreps was a pool of three plants. The field was located in central Illinois

表达AtBBX32的转基因大豆植株株高更高，节数、花量、荚果数及种子产量均显著提升。AtBBX32的表达调控效应在清晨时段对部分基因的表达影响最为显著。具体而言，AtBBX32可调控大豆生物钟基因（clock gene）TOC1与LHY1的表达。本实验在田间种植条件下设置5个时间点，对比了AtBBX32转基因植株与对照植株的响应差异。实验共包含4个株系、5个采样时间点，每组设置3次生物学重复，总计60份样本。4个株系中，3个为AtBBX32转基因株系（其中2个为高产株系[Event1与Event2]，1个为非高产株系[Event4]），剩余1个为非转基因对照株系[A3244]。采样时间为凌晨3点、6点、9点、正午与下午3点，对应生物钟时间（Zeitgeber Time，ZT）ZT21、ZT0、ZT3、ZT6与ZT9（日出时间为6:08，日落时间为20:08）。发育阶段与组织：R5期的三出复叶（出苗后80天）。每份生物学重复样本均为3株植株的混合样品。试验田位于伊利诺伊州中部。