H1.Kc

modENCODE_submission_5134 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: Kc167; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Genotype: se/e; Sex: Female; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line Kc167; Antibody H1 (target is H1)

modENCODE_submission_5134 本次提交来自Gary Karpen主导的modENCODE项目。如需查阅modENCODE项目的完整清单，请访问http://www.genome.gov/26524648。项目目标：本研究旨在定位黑腹果蝇（Drosophila melanogaster）基因组内主要组蛋白修饰的分布位点。所研究的修饰参与DNA复制、基因表达、基因沉默与遗传等核心染色体生物学功能。本研究将采用染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）结合基因组平铺阵列（genomic tiling arrays）的技术方案，初期将利用3种细胞系与2个胚胎阶段的染色质完成定位检测，后续将针对部分蛋白质的分析拓展至另外4种动物组织/发育阶段。关于数据使用的条款与细则，请参阅http://www.genome.gov/27528022 及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。实验类型：ChIP-chip。生物样本来源：细胞系：Kc167；组织类型：胚胎来源细胞系；发育阶段：晚期胚胎阶段；基因型：se/e；性别：雌性；重复次数：4次；实验因子：Kc167细胞系；H1抗体（靶标为H1组蛋白）