Raptor determines ß-cell identity and plasticity independent of metabolic state

In this study, we employed RNA-seq technology to identify the exact role of mTORC1 on ß-cell identity and plasticity. Overall design: ß cell specific Raptor deficiency induced gene expression from purified ß cells in mice with or without insulin pellets was measured at 4 weeks after the pellet implanted. ß cells from WT mice were set as controls.

本研究采用RNA测序（RNA-seq）技术，明确了雷帕霉素靶蛋白复合物1（mTORC1）在β细胞（β-cell）身份维持与细胞可塑性中的确切作用。实验整体设计如下：对植入与未植入胰岛素颗粒（insulin pellets）的小鼠，分离纯化其β细胞，检测由β细胞特异性Raptor蛋白缺陷诱导的基因表达水平，检测时间为颗粒植入后4周；以野生型（WT）小鼠的β细胞作为对照。