RIF sequences for Xanthomonas, Ralstonia and Clavibacter.

RIF and ITS marker characteristics for a set of 818 strains from the PBC and ICMP collections. Sequence contigs are assembled from read pairs.a-Total strains include characterized strains (separated from the total in parentheses) and unknown accessions.b- One strain did not produce a RIF amplicon that was visible on an agarose gel but did produce sequence data.c- the 191 strains only include those that produced an egl amplicon (Supplemental Table S2). RIF was sequenced in both directions more often (183/191 = 95.8%) than the egl gene (159/191 = 83.2%) for the same strains (Supplemental Table S2).d- Of the 32 failed sequencing reactions with egl 19 had a single direction read.*- Computed using a single representative for each unique sequence.

本数据集包含PBC与ICMP保藏中心的818株菌株的RIF及ITS标记特征。测序读段对经组装得到序列重叠群（sequence contig）。
a. 总菌株数包含已表征菌株（括号内为与总菌株分离的已表征菌株子集）与未知保藏菌株。
b. 有1株菌株未获得可在琼脂糖凝胶上检出的RIF扩增产物，但成功获取了测序数据。
c. 本次纳入分析的191株菌株仅包含成功扩增得到egl基因片段的菌株（详见补充表S2）。针对上述191株菌株，RIF标记的双向测序完成率（183/191，即95.8%）高于egl基因的双向测序完成率（159/191，即83.2%），相关统计结果详见补充表S2。
d. 在egl基因的32次测序失败反应中，有19次仅获得了单端测序读段。
* 该统计以每个唯一序列的单条代表序列为基准计算得到。