RNA-seq of Escherichia coli MG1655 WT strain against strain deleted of the ettA gene and complemented strain. RNA-seq of Escherichia coli MG1655 WT strain against strain deleted of the ettA gene and complemented strain

Comparison of transcripts of the WT strain versus the strain deleted of the ettA gene in condition where the deleted strain show a difference in growth rate compared to the WT strain. We also include a complemented strain where the gene ettA was inserted at the P21 locus in the etta deleted strain. Strains were cultivated in MJ9 medium with amino acids as carbon sources and with 0.4 M of NaCl.

本研究针对野生型（Wild Type, WT）菌株与敲除ettA基因的菌株，在二者生长速率存在差异的培养条件下开展转录本比较分析。此外，本研究还纳入了一株互补菌株：即在ettA敲除菌株的P21位点插入ettA基因所构建的工程菌株。所有菌株均在以氨基酸为碳源、添加0.4 M NaCl的MJ9培养基中进行培养。