Grapevine leaves Metagenome. leaf metagenome

Next generation sequencing technology was used to identify etiological agent(s) associated with grapevine redleaf disease (GRD). High quality RNA extracted from leaves of grapevine cultivars Merlot and Cabernet Franc with and without GRD symptoms were used to prepare cDNA libraries for Illumina sequencing.

本研究采用下一代测序技术（Next generation sequencing），对与葡萄红叶病（GRD）相关的病原物进行了鉴定。从带有或不带有GRD症状的梅鹿辄（Merlot）与品丽珠（Cabernet Franc）葡萄品种的叶片中提取的高质量RNA，被用于构建cDNA文库以开展Illumina测序。