Pro-inflammatory cytokine expression.
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Quantitative RT-PCR analysis of pro-inflammatory cytokine and COX-2 expression in human colon biopsies. Assessment of TNF-α, IFN-γ, IL-1β and COX-2 mRNA levels in healthy subjects without (Ctrl) or with a prior history (Hx) of UC, UC patients with active disease (active), or UC patients in medically-induced remission. Data were normalized to β-actin gene expression (n = 5–20; *PP
人类结肠活检组织中促炎细胞因子与环氧合酶-2（COX-2）表达的实时定量逆转录聚合酶链反应（Quantitative RT-PCR）分析。针对无溃疡性结肠炎（Ulcerative Colitis，UC）既往史的健康受试者（Ctrl，对照组）、有UC既往史者（Hx）、活动性溃疡性结肠炎患者以及经药物诱导缓解的溃疡性结肠炎患者，检测其肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、白细胞介素-1β（IL-1β）与COX-2的信使核糖核酸（mRNA）水平。所有数据均以β-肌动蛋白（β-actin）基因表达作为内参进行归一化处理（样本量n=5~20；*PP
创建时间：
2015-12-02
