Vulliard2021_BioProfilingJl

# High-content imaging chemical screen in U-2-OS<br><br>This dataset is described in the manuscript untitled "BioProfiling.jl: Profiling biological perturbations with high-content imaging in single cells and heterogeneous populations" by Vulliard _et al_ (DOI: [10.1101/2021.06.18.448961](https://doi.org/10.1101/2021.06.18.448961).<br>The following files are made available:<br><br>* `README.md`: description of the dataset.<br>* `750CellsPerWell.zip`: raw fluorescent images (TIFF) for a 384-well drug plate seeded with 750 cells per well.<br>* `1500CellsPerWell.zip`: raw fluorescent images (TIFF) for a 384-well drug plate seeded with 1500 cells per well.<br>* `Batch_data_u2os_drug.cpproj`: CellProfiler 3.1.8 pipeline used to analyse the images.<br>* `data.zip`: morphological measurements from CellProfiler per cell for each plate and plate layout. Necessary input to reproduce our [example analyses](https://github.com/menchelab/BioProfilingNotebooks).* `IntermediateResults.zip`: files generated in the [example analyses](https://github.com/menchelab/BioProfilingNotebooks).that are not required but helpful to avoid rerunning all scripts in order.<br><br>The preparation of the drug plates is described more precisely in the manuscript.

# U-2-OS细胞高内涵成像（high-content imaging）化学筛选数据集

本数据集对应Vulliard等人发表的题为《BioProfiling.jl：基于高内涵成像的单细胞与异质性群体的生物学扰动分析》的研究手稿，DOI：[10.1101/2021.06.18.448961](https://doi.org/10.1101/2021.06.18.448961)。

本次公开提供以下文件：

* `README.md`：数据集说明文档。
* `750CellsPerWell.zip`：每孔接种750个细胞的384孔药物培养板的原始荧光图像（TIFF格式）。
* `1500CellsPerWell.zip`：每孔接种1500个细胞的384孔药物培养板的原始荧光图像（TIFF格式）。
* `Batch_data_u2os_drug.cpproj`：用于图像分析的CellProfiler 3.1.8分析流程文件。
* `data.zip`：CellProfiler针对每个培养板及板布局输出的单细胞形态学测量数据，是复现我们的[示例分析](https://github.com/menchelab/BioProfilingNotebooks)的必要输入文件。
* `IntermediateResults.zip`：[示例分析](https://github.com/menchelab/BioProfilingNotebooks)中生成的中间结果文件，并非复现分析的必需文件，但可帮助用户避免重复运行全部脚本。

药物培养板的制备细节可参阅该手稿获取更精准的说明。