Dataset of Raman spectra and MATLAB code for: Elucidating time-resolved intracellular metabolic dynamics via label-free Raman microspectroscopy and 2D correlation spectroscopy

This dataset contains raw spectral data obtained from single-cell Raman microspectroscopy under two nutritional conditions: glucose alone and glucose supplemented with glutamine. LLC-MK2 cells were starved for 2 h, then exposed to nutrients and fixed every 15 min for up to 120 min. At each time point, spectra were recorded from 25 individual cells (cytoplasm regions) and exported as machine-readable files (.csv). MATLAB scripts are provided to implement two-dimensional correlation spectroscopy (2D-COS) for generating synchronous maps, along with utilities for loading spectra, averaging, and reproducing key figures. A set of simulated time-series spectra used to validate 2D-COS under high background interference is also included with code.本数据集包含两种营养培养条件下通过单细胞拉曼显微光谱术采集的原始光谱数据：仅添加葡萄糖组，以及葡萄糖添加谷氨酰胺组。实验中，LLC-MK2细胞先经过2小时饥饿处理，随后添加营养物质，并每隔15分钟固定一次样本，总时长最长达120分钟。在每个时间节点，研究人员从25个独立细胞的细胞质区域采集光谱，并将数据导出为机器可读的.csv格式文件。本数据集附带MATLAB脚本，可实现二维相关光谱（two-dimensional correlation spectroscopy, 2D-COS）分析以生成同步图谱，同时提供用于加载光谱数据、均值计算以及复现关键实验图表的配套工具。此外，数据集还包含一组模拟时间序列光谱数据及其配套代码，用于在高背景干扰环境下验证2D-COS的分析性能。