Multimodal profiling of lung granulomas in macaques reveals cellular correlates of tuberculosis control

We used longitudinal scRNA-seq, PET-CT, and bacterial burden assays to understand the cellular features underlying granuloma timing and burden/resolution. Overall design: Granulomas in Cynomolgus macaque were dissociated for scRNA-seq

本研究采用纵向单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）、正电子发射计算机断层显像（Positron Emission Computed Tomography-Computed Tomography, PET-CT）及细菌负荷检测实验，旨在解析肉芽肿形成时序、负荷状态与消退过程背后的细胞特征。实验总体设计：从食蟹猴体内分离肉芽肿组织并进行解离，以用于单细胞RNA测序。