carprofen storage data for repository.xlsx

The objective of this study was to evaluate the stability of carprofen in canine plasma when stored at -80oC for 6 months. Six healthy dogs were enrolled (1-10 years old, 15-50 kg) and received compounded carprofen at 2.2 mg/kg orally every 12 hours for 2 days. On the third day, blood was collected immediately before the morning dose (trough), then 1 and 6 hours after the dose (sampling timepoint). Whole blood was immediately centrifuged, and plasma was stored at –80oC. Plasma carprofen concentration was measured at multiple intervals between 2 days and 6 months (storage timepoint) using reversed phase high performance liquid chromatography. There was no effect of storage time over 6 months (p=0.891) on measured carprofen levels. While there was an effect of sampling timepoint (0, 1, 6 hours) (p<0.001), the interaction between storage timepoint and sampling timepoint was not statistically significant (p=1). Therefore, carprofen-laden canine plasma samples can be stored for up to 6 months before analysis with no degradation in carprofen concentrations expected.

本研究旨在评估卡洛芬（carprofen）在-80℃储存6个月时的犬血浆稳定性。招募6只健康犬（年龄1~10岁，体重15~50 kg），以2.2 mg/kg的剂量经口给予调配后的卡洛芬制剂，每12小时给药1次，连续给药2天。给药第3天，于晨间给药前（谷浓度时间点）、给药后1小时及6小时采集血液样本。全血样本经立即离心分离血浆后，将血浆置于-80℃条件下储存。采用反相高效液相色谱法，在2天至6个月的多个储存时间点检测血浆中卡洛芬的浓度。6个月储存期内，储存时间对卡洛芬的检测浓度无显著影响（p=0.891）。尽管采样时间点（0、1、6小时）对检测结果存在显著影响（p<0.001），但储存时间点与采样时间点的交互作用无统计学意义（p=1）。因此，含卡洛芬的犬血浆样本可在-80℃储存最长6个月后再进行分析，预期卡洛芬浓度不会发生降解。