Ribosome footprint profiling of the CHO cell response to reduced cell culture temperature

A study of translation initiation and elongation in a Chinese hamster ovary (CHO) cell line using ribosome footprint profiling (Ribo-seq). For this work, Ribo-seq was conducted with three different translation inhibitors along with RNA-seq to improve the characterisation of proteoforms and understand the impact of sub-physiological temperature on the CHO cell biological system.

本研究采用核糖体印迹测序（ribosome footprint profiling, Ribo-seq）技术，针对中国仓鼠卵巢（Chinese hamster ovary, CHO）细胞系的翻译起始与延伸过程展开探究。本次实验中，研究人员联合使用三种不同的翻译抑制剂开展核糖体印迹测序，并配合RNA测序（RNA-seq），以完善蛋白质亚型的表征鉴定，同时解析亚生理温度对CHO细胞生物学系统的影响。