Overview of the genotype and first-cycle phenotype of EAV nsp1 mutants described in this study.

aNucleotide substitutions are indicated in bold italics; numbers indicate the start and end coordinates of the mutated codons in the EAV genome.bTransfected cells were analyzed by IFA and gel hybridization analysis at 11 h post-transfection. Wild-type levels of replication and transcription (based on accumulation levels of genomic and sg mRNAs, respectively) are indicated with +++. A 2- to 4-fold increase in genomic RNA levels as compared to wild-type is denoted by ++++, while an increase of >4-fold is shown as +++++. Likewise, a >2-fold increase or decrease in accumulation levels of at least two sg mRNA species is shown as ++++ and ++, respectively (see text for details).cNA, not applicable.

a. 核苷酸替换以斜体加粗形式标注；数字用于指示EAV基因组中突变密码子的起始与终止坐标。
b. 转染后11小时，通过间接免疫荧光试验（Indirect Immunofluorescence Assay，IFA）与凝胶杂交分析对转染细胞进行检测。野生型的复制与转录水平（分别以基因组RNA与亚基因组mRNA（subgenomic mRNA，sg mRNA）的积累量为评判依据）以“+++”标注。相较于野生型，基因组RNA水平提升2~4倍时记为“++++”，提升幅度超过4倍则记为“+++++”。同理，若至少两种sg mRNA的积累量出现大于2倍的上调或下调，分别以“++++”和“++”标注（详细说明参见正文）。
c. NA：不适用。