Exploring the underlying mechanism by which circHNRNPU_603aa exerted carcinogenesis and its potential targets in MM

A RIP experiment was performed in CAG WT and CAG circHNRNPU_603aa OE cells using HA antibody as bait. The position of peak summit around transcript start sites of genes can predict the interaction sites of protein and gene. We compared the peak summit of CAG WT and CAG circHNRNPU_603aa OE cells to find the differences between CAG WT cells and CAG circHNRNPU_603aa cells. Examination of CAG EV and circHNRNPU_603aa OE cells.

以HA抗体作为捕获诱饵，在CAG野生型（WT）细胞与CAG circHNRNPU_603aa过表达（OE）细胞中开展了RNA免疫沉淀（RIP）实验。基因转录起始位点周边的峰顶点位置，可用于预测蛋白质与基因的互作位点。本研究比对了CAG WT细胞与CAG circHNRNPU_603aa OE细胞的峰顶点，以分析二者的差异。此外，本研究还对CAG空载体（empty vector, EV）细胞与circHNRNPU_603aa过表达细胞进行了检测。