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Studied genes, DNA sequences for forward and reverse primers (5΄-3΄), GenBank accession numbers, the PCR product length and approximate amplification efficiency.

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https://figshare.com/articles/dataset/_Studied_genes_DNA_sequences_for_forward_and_reverse_primers_5_900_3_900_GenBank_accession_numbers_the_PCR_product_length_and_approximate_amplification_efficiency_/1402530
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资源简介:
aADIPOQ, adiponectin; ADIPOR1, adiponectin receptor 1; ADIPOR2, adiponectin receptor 2; INSR, insulin receptor; LEP, leptin; MCP-1, monocyte chemoattractant protein-1; MRPL39, mitochondrial ribosomal protein L39; RBP4, retinol binding protein 4; SCD, stearoyl CoA desaturase; TNF, tumour necrosis factor-alpha. bFor, forward; Rev, reverse; exon-exon junctions are underlined. cGenBank accession numbers are from the databases of the National Center for Biotechnology Information; all sequences were derived from horse (Equus caballus). dE, approximate amplification efficiency for each gene calculated from amplification curves as described by Selim et al. [30]. Studied genes, DNA sequences for forward and reverse primers (5΄-3΄), GenBank accession numbers, the PCR product length and approximate amplification efficiency.

a. 脂联素(adiponectin):ADIPOQ;脂联素受体1(adiponectin receptor 1):ADIPOR1;脂联素受体2(adiponectin receptor 2):ADIPOR2;胰岛素受体(insulin receptor):INSR;瘦素(leptin):LEP;单核细胞趋化蛋白-1(monocyte chemoattractant protein-1):MCP-1;线粒体核糖体蛋白L39(mitochondrial ribosomal protein L39):MRPL39;视黄醇结合蛋白4(retinol binding protein 4):RBP4;硬脂酰辅酶A去饱和酶(stearoyl CoA desaturase):SCD;肿瘤坏死因子-α(tumour necrosis factor-alpha):TNF。 b. 注:For表示正向(forward)引物,Rev表示反向(reverse)引物;外显子-外显子剪接连接位点以下划线标注。 c. 注:GenBank登录号源自美国国家生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库;所有序列均取自马(Equus caballus)。 d. 注:E为参照Selim等[30]所述方法,通过扩增曲线计算得到的各基因近似扩增效率。 本数据集涵盖研究涉及的基因、正向与反向引物的DNA序列(5΄-3΄方向)、GenBank登录号、聚合酶链式反应(PCR)产物长度以及近似扩增效率。
创建时间:
2015-05-04
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