NeuCode metabolic labeling reveals a high correlation between mRNA and protein abundance during salt adaptation in S. cerevisiae.. Saccharomyces cerevisiae

Transcriptome profiling of yeast strains responding to 0.7M NaCl treatment for 30 or 60 minutes. This study identified affected genes under both conditions tested. Transcript and protein changes were compared to determine mRNAs contribution to protein levels during salt acclimation. Overall design: Two-color fluorescence arrays reporting on mRNA abunance in strains before and at 30 min or 60 min after a shock with 0.7M NaCl, hybridized to yeast tile-genome Nimblegen arrays

本研究针对经0.7摩尔/升氯化钠（NaCl）处理30分钟或60分钟的酵母菌株开展转录组分析（Transcriptome profiling），鉴定了两种测试条件下的受影响基因。本研究同时对比转录本与蛋白质的表达变化，以解析盐适应过程中信使RNA（mRNA）对蛋白质水平的贡献。实验整体设计如下：采用与Nimblegen公司酵母全基因组瓦片阵列芯片杂交的双色荧光基因芯片，检测酵母菌株在0.7M氯化钠冲击处理前、处理后30分钟及60分钟的mRNA丰度。