NanoPyx - Figures' Data

<strong>a549_mt.tif </strong> Data used in Figure "Microscopy image processing workflow using NanoPyx methods". Fixed A549 cells immunolabelled against ß-tubulin with Alexa Fluor™ 647. Acquired using a Nanoimager microscope (Oxford Nanoimaging; ONI) equipped with a 100 × oil-immersion objective (Olympus 100x NA 1.45) Image size 10000x283x283 (pixel size 117 nm) .tif 16-bit <strong>huvec_nuclei.tif</strong> Data used in Figure "Comparative run times of multiple implementations of an algorithm, ran on a consumer-grade laptop and a professional workstation". Human Umbilical Vein Endothelial Cells (HUVEC) stained with DAPI Acquired in a Marianas spinning-disk confocal microscope. The objective used was a 63x oil (NA 1.4 oil, Plan-Apochromat, M27). Image size 100x512x512 .tif 16-bit<strong>a549_mt.tif</strong> 为图《使用NanoPyx方法的显微镜图像处理流程》中所用实验数据。实验样本为经Alexa Fluor™ 647免疫标记β微管蛋白的固定A549细胞，使用搭载奥林巴斯100×油浸物镜（NA 1.45）的Nanoimager显微镜（牛津纳米成像公司，ONI）采集。图像尺寸为10000×283×283（像素尺寸117 nm），格式为16位.tif文件。<strong>huvec_nuclei.tif</strong> 为图《多种算法实现的运行时间对比（在消费级笔记本与专业工作站上运行）》中所用实验数据。实验样本为经DAPI染色的人脐静脉内皮细胞（Human Umbilical Vein Endothelial Cells, HUVEC），使用Marianas转盘共聚焦显微镜采集，所用物镜为63×油浸物镜（Plan-Apochromat M27，NA 1.4油镜）。图像尺寸为100×512×512，格式为16位.tif文件。